Questionnaire

1. Do you keep Cephalopods for: *

Culture: keeping animals’ trough out an entire life cycle;

Rearing: keeping a cephalopod for more then one life stage but not for all of its life cycle;

Maintenance: keeping a cephalopod for a single life stage.

2. What is the type of aquarium/tank system you are using? *

RAS (closed)RAS (semi-closed)Open-flowNatural sea-waterArtificial sea-waterOther

If OTHER, please specify:

2.1. What is the material utilized for tanks you use for Cephalopods? *

PVCGlassOther

If OTHER, please specify:

3. How many different species of cephalopods do you hold in your facility annually? *

4. Please list the species and their source (select any from: wild caught, eggs, captive bred animals) *

4.1. What is the most common method utilized for capture *

4.2. Do you have access to trained competent personnel for capture? *

YesNo

Please specify: *

5. How many individual cephalopods do you hold in your facility annually? *

6. Which type of food do you use to feed your animal? *

6.1. Please specify which type of food you use to feed your animal: *

7. Are you holding larval/juvenile stages? *

7.1. Please provide a short description (or reference) for the method utilized for keeping larval/juvenile stages: *

7.2. Please provide an estimated maximum time for keeping larval/juveniles in your facility/institute: *

8. Which parameters do you check and how often? *

TemperatureOxygenpHCarbon dioxideSalinityNH4NO2NO3Metals

Please add frequency: *

9. Do you anesthetize cephalopods? *

9.1. If YES, please specify how:

10. Do you euthanize cephalopods? *

10.1. If YES, please specify how:

11. Has your facility/institute a designated veterinarian? *

11.1. Has he/she experience with Cephalopods? *

11.2. Has he/she experience with other aquatic organisms? *

12. Do you transport cephalopods? *

12.1 If yes do you have data on water parameters utilized/monitored and survival rate?

13. Do you engage in research requiring a permission under the new European Directive 2010/63/EU? *

14. Do you know about the ARRIVE guidelines? *

14.1. If yes, please provide a very short description and comment about it

15. If you want, you can upload a file with further data/descriptions

16. Do you want to keep your answers anonymous? *

16.1. If NO, please write your email address here. We will contact you if more data or info are required